STAM2 Overexpression Lysate (Native)

Images

 
Western Blot: STAM2 Overexpression Lysate (Adult Normal) [NBL1-16510] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for STAM2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

STAM2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206279 and based on accession number NM_005843. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2), mRNA.
Gene
STAM2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for STAM2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for STAM2 Overexpression Lysate (Native)

  • DKFZp564C047
  • Hbp
  • Hrs-binding protein
  • HSE1 homolog
  • signal transducing adapter molecule 2
  • signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
  • STAM-2
  • STAM2A
  • STAM2B
  • STAM-like protein containing SH3 and ITAM domains 2

Background

The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for STAM2 Lysate (NBL1-16510) (0)

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Bioinformatics

Gene Symbol STAM2