STAM-1 Antibody

Western Blot: STAM-1 Antibody [NBP3-20227] - (1ug/ml) staining of K562 (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STAM-1 Antibody Summary

• Description: Novus Biologicals Goat STAM-1 Antibody (NBP3-20227) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KTEKKTVQFSDD, from the internal region of the protein sequence according to NP_003464.1; NP_001311216.1; NP_001311212.1; NP_001311215.1; NP_001311211.1; NP_001311217.1.
• Specificity: This antibody is expected to recognize usoforms a-f. No cross-reactivity expected with STAM2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STAM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3-1 ug/ml
• Application Notes: Western blot: Approx. 60kDa band observed in lysates of cell line K562, and approx. 55kDa in lysates of cell line MCF7, (calculated MW of 59.2kDa according to NP_003464.1 and 55.7kDa according to NP_001311211.1). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAM-1 Antibody

• DKFZp686J2352
• HSE1 Homolog
• signal transducing adapter molecule 1
• signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• STAM
• STAM1
• STAM-1
• STAM1STAM-1

Background

STAM was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAM