STAG2 Antibody

Western Blot: STAG2 Antibody [NBP1-30472] - Western Blot and Immunoprecipitation: Whole cell lysate from HeLa and 293T cells. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody NBP1-30473.

Immunohistochemistry-Paraffin: STAG2 Antibody [NBP1-30472] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SA2 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

STAG2 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human stromal antigen 2 using the numbering given in entry NP_006594.3 (GeneID 10735).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 141 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAG2 Antibody

• bA517O1.1
• cohesin subunit SA-2
• DKFZp686P168
• SA-2
• SA2FLJ25871
• SCC3 homolog 2
• stromal antigen 2DKFZp781H1753

Background

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAG2 Antibody (NBP1-30472)(1)

Publication using NBP1-30472

• Subramanian S, Thoms J, Huang Y et al. Cell Type-Specific Regulation by a Heptad of Transcription Factors in Human Hematopoietic Stem and Progenitor Cells bioRxiv 2023-04-19 (ChIP)
  
  Details:
  used 2µg per immunoprecipitation (IP)

Bioinformatics

• Gene Symbol: STAG2
• Entrez:
  • 10735(Human)
• Uniprot:
  • Q8N3U4(Human)