ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody

Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST6 Gal Sialyltransferase 2/ST6GAL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ST6 Gal Sialyltransferase 2/ST6GAL2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ST6 Gal Sialyltransferase 2/ST6GAL2
  Glu243-Ser529
  Accession # Q96JF0
• Specificity: Detects human ST6 Gal Sialyltransferase 2/ST6GAL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human ST6GAL1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ST6GAL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody

• Alpha 2,6-ST 2
• beta-galactoside alpha-2,6-sialyltransferase 2
• beta-galactoside alpha-2,6-sialyltransferase II
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
• EC 2.4.99
• EC 2.4.99.1
• FLJ30711
• FLJ37730
• FLJ38334
• hST6Gal II
• KIAA1877
• KIAA1877St6gal2
• sialyltransferase 2 (monosialoganglioside sialyltransferase)
• Sialyltransferase 2
• SIAT2
• ST6 beta-galactosamide alpha-2,6-sialyltranferase 2
• ST6Gal II
• ST6GAL2
• St6GalII

Background

ST6GAL2 (Sialyltransferase 2; also beta -galactoside alpha -2,6 Sialyltransferase 2) is a 60-62 kDa member of the glycosyltransferase 29 family of enzymes. It has restricted expression, likely being found in neurons and bronchial eipthelium. ST6GAL2 transfers sialic acid from CMP-Neu5Ac to a galactose that is linked to N‑Aceylglucosamine through a beta 1-4 linkage (Gal beta 1-4GlcNac). This transfer will occur when the target motif is found either on select glycoproteins or free oligosaccharides. Notably, the kinetics of this enzyme suggest additional biological functions other than sialic acid transfer. Human ST6GAL2 is a 529 amino acid (aa) type II transmembrane Golgi protein. It contains an 11 aa N‑terminal cytoplasmic region and a 497 aa luminal domain (aa 33-529). The luminal domain possesses an L motif that binds CMP-Sialic acid (aa 296-343), an S motif that binds CMP-Sialic acid and galactose (aa 444-458) and a VS motif that is involved with the enzyme's catalytic activity (aa 483-488). There is some evidence that ST6GAL2 may form homodimers. There is one splice variant that shows a 26 aa substitution for aa 441‑529. Over aa 243-529, human ST6GAL2 shares 88% aa sequence identity with mouse ST6GAL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody (AF7747)(4)

Publications using AF7747

• Kun Han, Feiyu Wang, Yulu Yue, Xihong Tan, Miao Tian, Yiliang Miao et al. Glycomics reveal that ST6GAL1‐mediated sialylation regulates uterine lumen closure during implantation Cell Proliferation 1/1/2022 [PMID: 34957619]
• Xun Tian, Xin Wang, Zifeng Cui, Jia Liu, Xiaoyuan Huang, Caixia Shi et al. A Fifteen‐Gene Classifier to Predict Neoadjuvant Chemotherapy Responses in Patients with Stage IB to IIB Squamous Cervical Cancer Advanced Science 5/1/2021 [PMID: 34026427]
• TR Karhadkar, D Pilling, N Cox, RH Gomer Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model Sci Rep, 2017-11-08;7(1):15069. 2017-11-08 [PMID: 29118338] (IHC-Fr, Human)
• Richard C, Doherty J, Fayad J, Cordero A, Linthicum F Identification of target proteins involved in cochlear otosclerosis. Otol Neurotol, 2015-06-01;36(5):923-31. 2015-06-01 [PMID: 25569361] (IHC, Human)

Bioinformatics

• Gene Symbol: ST6GAL2
• Uniprot:
  • Q96JF0(Human)