ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody [Alexa Fluor® 488]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ST6 Gal Sialyltransferase 2/ST6GAL2
  Glu243-Ser529
  Accession # Q96JF0
• Specificity: Detects human ST6 Gal Sialyltransferase 2/ST6GAL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human ST6GAL1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ST6GAL2 (Sialyltransferase 2; also beta -galactoside alpha -2,6 Sialyltransferase 2) is a 60-62 kDa member of the glycosyltransferase 29 family of enzymes. It has restricted expression, likely being found in neurons and bronchial eipthelium. ST6GAL2 transfers sialic acid from CMP-Neu5Ac to a galactose that is linked to N‑Aceylglucosamine through a beta 1-4 linkage (Gal beta 1-4GlcNac). This transfer will occur when the target motif is found either on select glycoproteins or free oligosaccharides. Notably, the kinetics of this enzyme suggest additional biological functions other than sialic acid transfer. Human ST6GAL2 is a 529 amino acid (aa) type II transmembrane Golgi protein. It contains an 11 aa N‑terminal cytoplasmic region and a 497 aa luminal domain (aa 33-529). The luminal domain possesses an L motif that binds CMP-Sialic acid (aa 296-343), an S motif that binds CMP-Sialic acid and galactose (aa 444-458) and a VS motif that is involved with the enzyme's catalytic activity (aa 483-488). There is some evidence that ST6GAL2 may form homodimers. There is one splice variant that shows a 26 aa substitution for aa 441‑529. Over aa 243-529, human ST6GAL2 shares 88% aa sequence identity with mouse ST6GAL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.