ST3GAL6 Antibody

Images

 
Western Blot: ST3GAL6 Antibody [NBP1-77353] - HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: ST3GAL6 Antibody [NBP1-77353] - HeLa cells with ST3GAL6 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ST3GAL6 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ST3gal6. The immunogen is located within amino acids 230 - 280 of ST3gal6.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ST3GAL6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Control Peptide
ST3GAL6 Peptide (NBP1-77353PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ST3GAL6 Antibody

  • alpha2,3-sialyltransferase
  • CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI
  • EC 2.4.99
  • EC 2.4.99.-
  • EC 2.4.99.9
  • Sialyltransferase 10
  • SIAT10
  • ST3 beta-galactoside alpha-2,3-sialyltransferase 6
  • ST3Gal VI
  • ST3GAL6
  • ST3GALVI
  • ST3GALVIsialyltransferase 10 (alpha-2,3-sialyltransferase VI)
  • type 2 lactosamine alpha-2,3-sialyltransferase

Background

Sialyltransferases catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures, such as the sialyl-Lewis X determinants, and are widely distributed in many cell types. However, cancer cells often express more heavily sialylated glycans on their cell surface and this feature sometimes correlates with invasiveness. In contrast, expression of ST3gal6, a member of the sialyltransferase family that sialylates type II lactosamine structures on glycoproteins and glycolipids, was found to be significantly decreased by hypermethylation of the gene in gastrointestinal cancer. At least three isoforms of ST3gal6 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ST3GAL6
Entrez
Uniprot