beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody

Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human prostate.

Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cerebral cortex, liver, prostate and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).

Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human liver.

Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human testis.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKM
• Specificity: Specificity of human beta-1,4-Galactosyltransferase 1/B4GalT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B4GALT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control:

  beta-1,4-Galactosyltransferase 1/B4GalT1 Knockout HeLa Cell Lysate

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23511973).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody

• 1,4- galactosyltransferase, polypeptide 1
• B4GalT1
• B4GAL-T1
• beta-1,4-galactosyltransferase 1
• Beta-1,4-GalTase 1
• beta4Gal-T1
• betaGlcNAc beta
• CDG2D
• EC 2.4.1
• GGTB2
• GGTB2DKFZp686N19253
• glycoprotein-4-beta-galactosyltransferase 2
• GT1
• GTB
• lactose synthase
• MGC50983
• UDP-Gal
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody (NBP1-88654)(1)

Publication using NBP1-88654

• Stalder L, Heusermann W, Sokol L et al. The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing. EMBO J 2013 Apr 17 [PMID: 23511973]

Bioinformatics

• Gene Symbol: B4GALT1