SREC-I/SCARF1 Antibody (373606) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: Flow
• Clone: 373606
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

SREC-I/SCARF1 Antibody (373606) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SREC‑I/SCARF1
  Ser20-Thr421
  Accession # Q14162
• Specificity: Detects human SREC‑I/SCARF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SREC-2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCARF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SREC-I/SCARF1 Antibody (373606) [Alexa Fluor® 350]

• Acetyl LDL receptor
• KIAA0149endothelial cells scavenger receptor
• MGC47738
• SCARF1
• scavenger receptor class F, member 1
• Scavenger receptor expressed by endothelial cells 1
• SREC1
• SRECI
• SREC-I
• SRECscavenger receptor expressed by endothelial cells
• SR-F1

Background

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1‑3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I; also called SCARF1) and SREC-2 are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain 8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain 8 plus domains 9 and 10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa and is expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region, human SREC-I shares 76% and 53% aa sequence identity with mouse SREC-I and human SREC-2, respectively.

1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCARF1
• Uniprot:
  • Q14162()