SREBP1 Antibody [DyLight 405]

Images

 
There are currently no images for SREBP1 Antibody (NB100-60545V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

SREBP1 Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to a portion of the human SREBP1 protein sequence (between residues 700-800). [UniProt# P36956]
Localization
Integral membrane protein that moves from ER to Golgi in absence of sterols.
Predicted Species
Porcine (100%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SREBF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This SREBP1 antibody is useful for Western blot, where a band is seen at ~65 kDa (mature form).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, and hamster. Predicted to react with porcine based on 100% sequence homology. Immunogen sequence has 94% homology to bovine, 88% homology to Zebrafish and 70% homology to chicken.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SREBP1 Antibody [DyLight 405]

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • SREBP1bHLHd1SREBP-1c
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-54446
Species: Hu, Ch
Applications: WB, ELISA, GS, ICC/IF, IP
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-42632
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
AF6898
Species: Hu, Mu, Rt
Applications: WB
AF7550
Species: Hu
Applications: WB
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP1-91996
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB400-135
Species: Hu, Mu, Rt
Applications: WB, ChIP, IB, ICC/IF, IHC, IHC-P, IP
NB100-60545V
Species: Hu, Mu, Rt, Ha, Po, Bv
Applications: WB

Publications for SREBP1 Antibody (NB100-60545V) (0)

There are no publications for SREBP1 Antibody (NB100-60545V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SREBP1 Antibody (NB100-60545V) (0)

There are no reviews for SREBP1 Antibody (NB100-60545V). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB100-60545V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SREBP1 Products

Bioinformatics Tool for SREBP1 Antibody (NB100-60545V)

Discover related pathways, diseases and genes to SREBP1 Antibody (NB100-60545V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SREBP1 Antibody (NB100-60545V)

Discover more about diseases related to SREBP1 Antibody (NB100-60545V).
 

Pathways for SREBP1 Antibody (NB100-60545V)

View related products by pathway.

PTMs for SREBP1 Antibody (NB100-60545V)

Learn more about PTMs related to SREBP1 Antibody (NB100-60545V).
 

Research Areas for SREBP1 Antibody (NB100-60545V)

Find related products by research area.

Blogs on SREBP1.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SREBP1 Antibody [DyLight 405] and receive a gift card or discount.

Bioinformatics

Gene Symbol SREBF1