SR-AI/MSR Antibody (351615) [Unconjugated]

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SR-AI/MSR Monoclonal Antibody (Catalog # MAB2708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR-AI/MSR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

M2 macrophages cells treated with 50 ng/mL Recombinant Human M-CSF Protein (216-MC) for 6 days followed by an overnight polarization with 20 ng/mL Recombinant Human IL-4 Protein (204-IL) and 20 ng/mL Recombinant Human IL-13 Protein (213-ILB) were stained with Mouse Anti-Human SR‑AI/MSR Monoclonal Antibody (Catalog # MAB2708, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 351615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SR-AI/MSR Antibody (351615) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSR
  Lys77-Leu451
  Accession # P21757
• Specificity: Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SR-AI/MSR Antibody (351615) [Unconjugated]

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-AI/MSR Antibody (MAB2708)(7)

Publications using MAB2708

• Duomeng Yang, Tao Lin, Cen Li, Andrew G. Harrison, Tingting Geng, Penghua Wang A critical role for MSR1 in vesicular stomatitis virus infection of the central nervous system iScience 6/25/2021 [PMID: 34169243]
• Long Yang, Tingting Geng, Guang Yang, Jinzhu Ma, Leilei Wang, Harshada Ketkar et al. Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice Communications Biology 10/8/2020 [PMID: 33033362]
• Niklas Mejhert, Leena Kuruvilla, Katlyn R. Gabriel, Shane D. Elliott, Marie-Aude Guie, Huajin Wang et al. Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression Molecular Cell 3/1/2020 [PMID: 32023484]
• Johanna Wagner, Maria Anna Rapsomaniki, Stéphane Chevrier, Tobias Anzeneder, Claus Langwieder, August Dykgers et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer Cell 5/16/2019 [PMID: 30982598]
• Johanna Wagner, Maria Anna Rapsomaniki, Stéphane Chevrier, Tobias Anzeneder, Claus Langwieder, August Dykgers et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer Cell 2019-05-16 [PMID: 30982598] (Immunocytochemistry, Human)
• Stéphane Chevrier, Jacob Harrison Levine, Vito Riccardo Tomaso Zanotelli, Karina Silina, Daniel Schulz, Marina Bacac et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma Cell 5/4/2017 [PMID: 28475899]
• Hongo S, Watanabe T, Arita S Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am. J. Physiol. Endocrinol. Metab., 2009-08-01;297(2):E474-82. 2009-08-01 [PMID: 19625677] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MSR1
• Uniprot:
  • P21757()