SPINK5 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human SPINK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8515) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SPINK5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SPINK5 Antibody [Unconjugated] Summary

• Immunogen: Human embyronic kidney cell line HEK293-derived recombinant human SPINK5
  Glu626-Glu1064
  Accession # Q9NQ38
• Specificity: Detects human SPINK5 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPINK5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPINK5 Antibody [Unconjugated]

• Kazal type 5
• LEKTI
• LETKI
• NETS
• serine peptidase inhibitor, Kazal type 5
• SPINK5
• VAKTI

Background

LEKTI (Lympho-epithelial Kazal-type-related inhibitor; also SPINK5, Serine Protease Inhibitor Kazal-type 5) is a 150 kDa precursor protein that is cleaved into the following 2 chains: Hemofiltrate peptide HF6478 and Hemofiltrate peptide HF7665. LEKTI is a multidomain serine protease inhibitor that contains 15 Kazal-like inhibitory domains. It may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. It contains fifteen Kazal-like domains. Mature human SPINK5 shares 60% aa sequence identity with mouse SPINK5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPINK5 Antibody (AF8515)(1)

Publication using AF8515

• Xiaoying Dong, Xuyuan Chen, Di Lu, Dingwei Diao, Xiguang Liu, Shijie Mai et al. LncRNA miR205HG hinders HNRNPA0 translation: anti‐oncogenic effects in esophageal carcinoma Molecular Oncology 2022-02-01 [PMID: 34821009]

Bioinformatics

• Gene Symbol: SPINK5
• Uniprot:
  • Q9NQ38()