Spi-B Antibody (769653) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clone: 769653
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Spi-B Antibody (769653) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Spi-B
  Ala7-Leu115
  Accession # Q01892
• Specificity: Detects human Spi-B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spi-B is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spi-B Antibody (769653) [Alexa Fluor® 405]

• Spi-B transcription factor (Spi-1/PU.1 related)
• SpiB
• Spi-B
• transcription factor Spi-B

Background

Spi-B is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. The 262 amino acid (aa) human Spi-B contains a dual transactivation region (aa 1-62) and an ets DNA-binding domain (aa 169-252). One 177 aa variant diverges after aa 162. Over aa 7-115, human Spi-B shares 72% and 70% aa identity with mouse and rat Spi-B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.