Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Western Blot: Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody [NBP3-23393] - Western blot of human recombinant Sphingosine Kinase 2/SPHK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-Sphingosine Kinase 2/SPHK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-Sphingosine Kinase 2/SPHK2 (Thr-578) (lanes 3, 4, 7, & 8).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Sphingosine Kinase 2/SPHK2 (Thr-578) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-578 in human Sphingosine Kinase 2/SPHK2. This peptide sequence is highly conserved in rat and mouse Sphingosine Kinase 2/SPHK2 proteins, and is not well conserved in SK1. [UniProt# Q9NRA0]
• Modification: p Thr578
• Specificity: The antibody detects 70 kDa proteins corresponding to the molecular mass of Sphingosine Kinase 2/SPHK2 on SDS-PAGE immunoblots of human recombinant Sphingosine Kinase 2/SPHK2 and endogenous Sphingosine Kinase 2/SPHK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

• EC 2.7.1.91
• SK 2
• SK2
• sphingosine kinase 2
• sphingosine kinase type 2 isoform
• SPHK2
• SPK 2
• SPK2

Background

SPHK2 catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK2