Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HeLa cells.

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human SPHK1(NP_068807) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPHK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:400-4000
• Theoretical MW: 43.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1