Sphingosine Kinase 1/SPHK1 Antibody (M540)

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: M540
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Sphingosine Kinase 1/SPHK1 Antibody (M540) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M540 was generated from a Sphingosine Kinase 1/SPHK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse Sphingosine Kinase 1/SPHK1. This sequence has high homology to human and rat Sphingosine Kinase 1/SPHK1, and is not homologous to sequences in Sphingosine Kinase 2/SPHK2. [UniProt# Q8CI15]
• Specificity: The antibody detects 42 kDa full-length recombinant human and mouse Sphingosine Kinase 1/SPHK1 proteins, as well as detects 42, 50, and 60 kDa forms of Sphingosine Kinase 1/SPHK1 in human HeLa cells.
• Clonality: Monoclonal
• Host: Rat
• Gene: SPHK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:250
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody (M540)

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1