Spectrin beta 3 Antibody (1287A) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1287A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 350

Spectrin beta 3 Antibody (1287A) [Alexa Fluor® 350] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing the C-terminal region of beta-III Spectrin
  Accession # NP_008877.1
• Specificity: Detects human beta-III Spectrin in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spectrin beta 3 Antibody (1287A) [Alexa Fluor® 350]

• beta-III Spectrin
• glutamate transporter EAAT4-associated protein 41
• GTRAP41
• KIAA0302
• SCA5
• SCAR14
• Spectrin beta 3
• spectrin beta chain, brain 2
• Spectrin beta III
• spectrin, beta, non-erythrocytic 2
• Spectrin, non-erythroid beta chain 2
• spinocerebellar ataxia 5
• SPTBN2

Background

Spectrin beta-III (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta-III interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.