SPARC-like 1/SPARCL1 Antibody [CoraFluor™ 1]

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

SPARC-like 1/SPARCL1 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human SPARC-like 1/SPARCL1 (Accession#: NP_004675.3; Met 1-Phe 664)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPARCL1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for SPARC-like 1/SPARCL1 Antibody [CoraFluor™ 1]

• Hevin
• High endothelial venule protein
• MAST 9
• MAST9
• PIG33
• proliferation-inducing protein 33
• SC1
• SPARC like 1
• SPARCL1
• SPARC-like 1 (hevin)
• SPARC-like 1 (mast9, hevin)
• SPARC-like 1
• SPARC-like protein 1

Background

Human SPARC-like 1 (SPARCL1), also known as MAST9 or Hevin, is a member of the SPARC protein family. It is a secreted acidic calcium-binding protein of 664 amino acids that shows 62% identity with SPARC. SPARCL1 is a secreted protein, found in the extracellular matrix. It is expressed at high levels in high endothelial venules (HEV) in lymphoid tissues. SPARCL1 inactivation is a common event in tumors of epithelial origin and levels are down-regulated in a variety of tumors. It may have tumor suppressor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPARCL1