SPARC Antibody


Western Blot: SPARC Antibody [NBP2-27371] - analysis of human A375 lysate probled with SPARC antibody at 2 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27371] - Formalin-fixed, paraffin-embedded human placenta stained with SPARC antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Product Details

Reactivity Hu, PmSpecies Glossary
Applications WB, IHC-P
0.5 mg/ml

Order Details

SPARC Antibody Summary

A portion of amino acids 150-210 of human SPARC was used as the immunogen for the antibody. (aa179-197)
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1 mg SPARC antibody in 0.2 ml PBS containing 0.05% BSA.
0.05% Sodium Azide
0.5 mg/ml
Protein A purified


Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human and monkey and 95% homolgous in mouse and rat.

Alternate Names for SPARC Antibody

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)


SPARC, also called Osteonectin, is an extracellular matrix (ECM) associated glycoprotein. SPARC activates matrix metalloproteinase-2, a key effector of ECM proteolysis, a process linked to enhanced tumor invasion and metastatic potential (1). MMP expression by tumor cells themselves may be representative of more invasive migratory cancers or latter stages of tumor progression (2, 3). SPARC appears to enhance MMP-2 activation at least in invasive breast cancer cell lines. Levels of SPARC expression appear to be directly correlated with progressive cancers not only of breast, but colon, kidney, ovary and brain (4, 5). SPARC specific antibody may establish levels of expression of SPARC providing its' use as a biomarker of late stage or aggressive tumors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


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Blogs on SPARC.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...  Read full blog post.

Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SPARC

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