SPARC Antibody (122511) [Unconjugated]

MG-63 cells were stained with Mouse Anti-Human SPARC Monoclonal Antibody (Catalog # MAB941, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human SPARC Monoclonal Antibody (Catalog # MAB941, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

SPARC/Osteonectin was detected in immersion fixed paraffin-embedded sections of human ovarian clear cell carcinoma tissue using Human SPARC/Osteonectin Monoclonal Antibody (Catalog # MAB941) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human VSIG8 protein was serially diluted 2-fold and captured by Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB9418) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA, IHC, CyTOF-ready, Flow
• Clone: 122511
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SPARC Antibody (122511) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SPARC
  Ala18-Ile303
  Accession # P09486
• Specificity: Detects human SPARC/Osteonectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SPARC/Osteonectin is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPARC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPARC Antibody (122511) [Unconjugated]

• Basement-membrane protein 40
• BM-40
• ONcysteine-rich protein
• Osteonectin
• Secreted protein acidic and rich in cysteine
• secreted protein, acidic, cysteine-rich (osteonectin)
• SPARC

Background

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 286 amino acid (aa), 43 kDa protein contains an N-terminal acidic region that binds calcium, a follistatin domain that contains Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure modeling shows that residues implicated in cell binding, inhibition of cell spreading, and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types undergoing an endothelial to mesenchymal transition; its expression, however, mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9-11). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (12). Mature human SPARC shows 92%, 92%, 97%, 99%, 96% and 85% aa identity with mouse, rat, canine, bovine, porcine and chick SPARC, respectively.

1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
2. Sweetwyne, M. T. et al. (2004) J. Histochem. Cytochem. 52:723.
3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
4. Framson, P. E. and E. H. Sage (2004) J. Cell. Biochem. 92:679.
5. Alford, A. I. and K. D. Hankenson (2006) Bone 38:749.
6. Hohenester, E et al. (1997) EMBO J. 16:3778.
7. Sage, E. H. et al. (2003) J. Biol. Chem. 278:37849.
8. Delany, A. M. et al. (2003) Endocrinology 144:2588.
9. Robert, G. et al. (2006) Cancer Res. 66:7516.
10. Koblinski, J. E. et al. (2005) Cancer Res. 65:7370.
11. Tai, I. T. et al. (2005) J. Clin. Invest. 115:1492.
12. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPARC Antibody (MAB941)(11)

Publications using MAB941

• Varga, N;Pomozi, V;Kozák, E;Erdei, Z;Tóth, S;Kutás, A;Mucsi, Z;Rapi, Z;Kovács, B;Matuscsák, A;Bánhídi, O;Váradi, C;Rácz, T;Német, K;Viskolcz, B;Szepesi, Á; Development and validation of combined in vitro and in vivo assays for evaluating the efficacy of strontium-chelating compounds Scientific reports 2025-11-28 [PMID: 41315304] (Immunocytochemistry, Human)
• Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB; PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms Cell reports 2024-07-23 [PMID: 39046873] (Western Blot, Human)
• Jessica S. Sadick, Michael R. O’Dea, Philip Hasel, Taitea Dykstra, Arline Faustin, Shane A. Liddelow Astrocytes and oligodendrocytes undergo subtype-specific transcriptional changes in Alzheimer’s disease Neuron 2022-06-01 [PMID: 35381189]
• Xiaobo Zhou, Lan Zhang, Caihang Qierang, Min Huang, Xin Yang, Liangang Li et al. Investigating the relationship between secreted protein acidic and rich in cysteine expression level and therapeutic efficacy of nab-paclitaxel: a meta-analysis Translational Cancer Research 2021-02-01 [PMID: 35116417]
• Yena Oh, Sibao Yang, Xueyan Liu, Sayantan Jana, Farzad Izaddoustdar, Xiaodong Gao et al. Transcriptomic Bioinformatic Analyses of Atria Uncover Involvement of Pathways Related to Strain and Post-translational Modification of Collagen in Increased Atrial Fibrillation Vulnerability in Intensely Exercised Mice Frontiers in Physiology 2020-12-23 [PMID: 33424629]
• A Baird, T Lindsay, A Everett, V Iyemere, YZ Paterson, A McClellan, FMD Henson, DJ Guest Osteoblast differentiation of equine induced pluripotent stem cells Biol Open, 2018-05-10;0(0):. 2018-05-10 [PMID: 29685993] (ICC, Equine)
• SH Mauzo, DR Milton, VG Prieto, CA Torres-Cab, WL Wang, N Chakravart, P Nagarajan, MT Tetzlaff, JL Curry, D Ivan, RE Brown, PP Aung Summary of expression of SPARC protein in cutaneous vascular neoplasms and mimickers Ann Diagn Pathol, 2018-03-15;34(0):151-154. 2018-03-15 [PMID: 29660567] (IHC-P, Human)
• Puyuan Xing, Yixiang Zhu, Ling Shan, Sipeng Chen, Xuezhi Hao, Junling Li The role of weekly nanoparticle albumin bound paclitaxel monotherapy as second line or later treatment for advanced NSCLC in China Oncotarget 2017-10-20 [PMID: 29152093]
• E Borazanci, SZ Millis, J Kimbrough, N Doll, D Von Hoff, RK Ramanathan Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis J Gastrointest Oncol, 2017-02-01;8(1):164-172. 2017-02-01 [PMID: 28280620] (IHC-P, Human)
• Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells. Glia, 2006-06-01;53(8):845-57. 2006-06-01 [PMID: 16586492] (ELISA Development, Human)
• Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell Proteomics, 2005-10-08;5(1):157-71. 2005-10-08 [PMID: 16215274] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SPARC
• Uniprot:
  • P09486()