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SPAG8 Antibody

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Western Blot: SPAG8 Antibody [H00026206-B01P] - Analysis of SPAG8 expression in human kidney.
Western Blot: SPAG8 Antibody [H00026206-B01P] - Analysis of SPAG8 expression in human kidney.
Western Blot: SPAG8 Antibody [H00026206-B01P] - Analysis of SPAG8 expression in transfected 293T cell line by SPAG8 polyclonal antibody. Lane 1: SPAG8 transfected lysate(55.11 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SPAG8 Antibody Summary

Immunogen
SPAG8 (AAH19247.1, 1 a.a. ~ 501 a.a) full-length human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SPAG8
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
This antibody is useful for Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPAG8 Antibody

  • BS-84
  • HSD-1MGC26201
  • hSMP-1
  • SMP1
  • SMP-1
  • SPAG3
  • sperm associated antigen 8
  • Sperm membrane protein 1
  • Sperm membrane protein BS-84
  • sperm-associated antigen 8

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lysate (Native)
NBP2-07087
Western Blot: RHD Overexpression Lysate (Adult Normal) [NBP2-07087] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RHD.
Species: Hu
RHCE Antibody
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Western Blot: RHCE Antibody [NBP1-69668] - This Anti-RHCE antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml.
Species: Hu
Applications: WB
POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Western blot analysis of POMC using NB100-1533 at 0.01ug/ml in NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Analysis in human Anterior Pituitary Gland shows vesilulate staining in glandular cells.
(7 Publications)
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
FHL5 Antibody
(1G12-D2)
H00009457-M01
Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01] - Analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2.Lane 1: FHL5 transfected lysate(32.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: FHL5 Antibody (1G12-D2) [H00009457-M01] - Analysis of monoclonal antibody to FHL5 on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01] - Western Blot analysis of FHL5 expression in HepG2 ( Cat # L019V1 ).
Species: Hu
Applications: WB, ELISA, ICC/IF
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Species: Mu
Applications: WB, ICC
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NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
(4 Reviews)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
Carbohydrate
Sulfotransferase 3/CH ...
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Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC
Corticotropin
Releasing Factor Anti
NBP2-41273
Western Blot: Corticotropin Releasing Factor Antibody [NBP2-41273] - Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: Corticotropin Releasing Factor Antibody [NBP2-41273] - Corticotropin Releasing Factor in human brain tissue with Corticotropin Releasing Factor antibody at 5 ug/mL.
Immunofluorescence: Corticotropin Releasing Factor Antibody [NBP2-41273] - Immunofluorescence of Corticotropin Releasing Factor in human brain tissue with Corticotropin Releasing Factor antibody at 20 ug/mL.
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
Aldo-keto
Reductase 1C1/AKR1C1 ...
MAB6529
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu
Applications: WB, Simple Western
FLIP Antibody
NB100-56141
Western Blot: FLIP Antibody [NB100-56141] - analysis of FLIP in human ovarian cancer cell lines using anti-FLIP antibody. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C in 5% milk in PBST. Image from verified customer review.
Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.
Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.
(1 Review)
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RANBP9 Antibody
NBP2-15087
Western Blot: RANBP9 Antibody [NBP2-15087] - Sample (30 ug of whole cell lysate) A: Hela B: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: RANBP9 Antibody [NBP2-15087] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Species: Hu, Mu
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GR/NR3C1 Antibody
(BuGR2)
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(2 Reviews)
(7 Publications)
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
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NBP2-37954
Immunocytochemistry/Immunofluorescence: HSD11B2 Antibody [NBP2-37954] - Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37954] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37954] - Staining of human kidney shows high expression.
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MUC-1 Antibody
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Western Blot: MUC-1 Antibody [NBP1-60046] - Total protein from mouse 3T3 cells and human HeLa cells, stomach and colon was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MUC-1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody [NBP1-60046] - MUC1 antibody (NBP1-60046) was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Cells were counterstained for alpha-tubulin against Dylight 550 (Red) and mounted in DAPI fluoromount (Blue).
Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.
(2 Publications)
Species: Hu, Mu, Rt, Po, Bv, Eq, Gt, GP, Rb
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Progesterone
R/NR3C3 Antibody [Unc ...
AF5415
Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
Species: Hu
Applications: WB, IHC, ICC
MEF2A Antibody
(JA33-04)
NBP2-66943
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human tonsil tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Serpin A6/Cortisol
Binding Globulin Anti ...
MAB3838
Western blot shows lysates of rat olfactory bulb tissue, rat liver tissue, rat brain (cerebellum) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A6 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A6 was detected in perfusion fixed frozen sections of rat brain (preoptic nucleus) using Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Species: Rt
Applications: WB, IHC
17
beta-HSD1/HSD17B1 Ant ...
MAB7178
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human 17  beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human 17 beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(1 Review)
Species: Hu
Applications: WB, Simple Western
HSD17B2 Antibody
NBP1-92011
Western Blot: HSD17B2 Antibody [NBP1-92011] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: HSD17B2 Antibody [NBP1-92011] - Staining of human cell line RT4 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining in human duodenum and cerebral cortex tissues using anti-HSD17B2 antibody. Corresponding HSD17B2 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Serpin A3/alpha
1-Antichymotrypsin An ...
NBP2-52559
Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis using SERPINA3 mAb against human SERPINA3 (AA: 279-432) recombinant protein. (Expected MW is 42.3 kDa)
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis of paraffin-embedded bladder cancer tissues using SERPINA3 mouse mAb with DAB staining.
Flow Cytometry: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis of A549 cells using SERPINA3 mouse mAb (green) and negative control (red).
(1 Publication)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready

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Bioinformatics

Gene Symbol SPAG8
Uniprot