SPAG6 Antibody - BSA Free

Western Blot: SPAG6 Antibody - BSA Free [NBP3-45855] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (SPAG6 antibody) at dilution of 1:300

Immunoprecipitation: SPAG6 Antibody - BSA Free [NBP3-45855] - IP Result of anti-SPAG6 (IP:, 3ug; Detection: 1:300) with mouse testis tissue lysate 4000ug.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPAG6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPAG6 Antibody - BSA Free (NBP3-45855) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: sperm associated antigen 6. (Uniprot# O75602)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPAG6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 50-55 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SPAG6 Antibody - BSA Free

• axoneme central apparatus protein
• DKFZp434I153
• pf16
• Protein PF16 homolog
• Repro-SA-1MGC26276
• sperm associated antigen 6
• Sperm flagellar protein
• sperm-associated antigen 6

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAG6