SOX10 Antibody (20B7) [NL557]

SOX10 was detected in immersion fixed Ntera2 cells using Mouse Anti-Human/Rat SOX10 NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL2864R) at a final concentration of 1X (1:10 dilution) for 3 hours at room temperature (A). Cells were counterstained with DAPI (blue, B). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Rt
• Applications: ICC/IF
• Clone: 20B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: NL557

SOX10 Antibody (20B7) [NL557] Summary

• Immunogen: E. coli-derived recombinant rat SOX10
  aa 1-118
• Specificity: Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:10 dilution

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied as a 10X solution of antibody in 0.5 mL PBS containing 0.1% sodium azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX10 Antibody (20B7) [NL557]

• DOM
• MGC15649
• PCWH
• SOX10
• SRY (sex determining region Y)-box 10
• SRY-related HMG-box gene 10
• transcription factor SOX-10
• WS4
• WS4C
• WS4mouse, human homolog of

Background

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

1. Lo, L. et al. (2002) Development 129:1553.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX10 Antibody (NL2864R)(2)

Publications using NL2864R

• G Shireby, EL Dempster, S Policicchi, RG Smith, E Pishva, B Chioza, JP Davies, J Burrage, K Lunnon, D Seiler Vel, S Love, A Thomas, K Brookes, K Morgan, P Francis, E Hannon, J Mill DNA methylation signatures of Alzheimer's disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types Nature Communications, 2022-09-24;13(1):5620. 2022-09-24 [PMID: 36153390] (Flow Cytometry, Human)
• Qinjie Weng, Jiajia Wang, Jiaying Wang, Biqin Tan, Jing Wang, Haibo Wang et al. Folate Metabolism Regulates Oligodendrocyte Survival and Differentiation by Modulating AMPK alpha Activity Scientific Reports 2017-05-11 [PMID: 28496133]

Bioinformatics

• Gene Symbol: SOX10