Recombinant Mouse Sonic Hedgehog/Shh Protein

Product Discontinued

Recombinant Mouse Sonic Hedgehog/Shh Protein Summary

• Description: Recombinant bioactive protein containing 176 amino acids for Mouse Sonic Hedgehog/Shh
  
  

  Source:E. coli
  Amino Acid Sequence:(Q8C765)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: Mouse SHH is fully biologically active when compared to standard. Determined by it's ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 0.03 -0.30 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SHH
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.01 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 10 mM sodium phosphate (pH 7.5).
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute the lyophilized product with sterile H2O to a concentration of 0.1 - 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh Protein

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SHH