Recombinant Mouse Sonic Hedgehog/Shh Protein

SDS-Page: Mouse Sonic Hedgehog/Shh Protein [NBP2-35174]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse Sonic Hedgehog/Shh Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Sonic Hedgehog/Shh Source: E. coli

  Uniprot ID: Q62226

  Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

• Details of Functionality: Sonic Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SHH
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Sonic Hedgehog/Shh as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh Protein

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Sonic Hedgehog (Shh) is a secreted protein that regulates developmental morphogenesis of the central nervous system, somite, limb, whisker and hair follicles, foregut, tooth, and bone. Shh is also involved in regulating neural and hematopoeitic stem cell fates and determination of the T cell repertoire. Aberrant Shh signaling is implicated in the development of several malignancies. Shh undergoes autocatalytic processing that yields a palmitoylated N-terminal fragment and a C-terminal fragment. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain acts as a cholesterol transferase. Shh activity is mediated by a multicomponent receptor complex that includes Patched and Smoothened. The N-terminal fragment of Shh is greater than 98% conserved between mouse, human, rat, canine, porcine, and chicken.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SHH
• Uniprot:
  • Q62226()
  • Q62226()
  • Q62226()