Sonic Hedgehog/Shh Antibody (171028) [Biotin] - N-Terminus

• Reactivity: Mu
• Applications: ELISA(Det)
• Clone: 171028
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

Sonic Hedgehog/Shh Antibody (171028) [Biotin] - N-Terminus Summary

• Immunogen: E. coli-derived recombinant mouse Sonic Hedgehog N-Terminus
  Cys25-Gly198 (Lys122Arg)
  Accession # Q62226
• Specificity: Detects mouse Sonic Hedgehog (Shh) N-terminal peptide in ELISAs. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human (rh) Shh N-terminal peptide but no cross-reactivity with rmDhh N-terminal (aa 23‑198) or C‑terminal (aa 199‑396) peptides, rmShh C-terminal peptide (aa 199-437), or rmHIP.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SHH
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse Sonic Hedgehog/Shh N-Terminus Antibody (Catalog # MAB4641)
  ELISA Detection: Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody (Catalog # BAM4642)
  Standard:Recombinant Mouse Sonic Hedgehog/Shh N-Terminus (Catalog # 461-SH)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sonic Hedgehog/Shh Antibody (171028) [Biotin] - N-Terminus

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sonic Hedgehog/Shh Antibody (BAM4642)(2)

Publications using BAM4642

• Céline Delloye-Bourgeois, Benjamin Gibert, Nicolas Rama, Jean-Guy Delcros, Nicolas Gadot, Jean-Yves Scoazec et al. Sonic Hedgehog Promotes Tumor Cell Survival by Inhibiting CDON Pro-Apoptotic Activity PLoS Biology 2013-08-06 [PMID: 23940460] (ELISA Detection, Mouse)
• Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development. Stem Cells, 2007-02-01;25(5):1156-65. 2007-02-01 [PMID: 17272496] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: SHH
• Uniprot:
  • Q62226()