SON Antibody

Western Blot: SON Antibody [NBP1-88706] - Analysis using Anti-SON antibody NBP1-88706 (A) shows similar pattern to independent antibody NBP2-55411 (B).

Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SON Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TEVAIESTPMILESSIMSSHVMKGINLSSGDQNLAPEIGMQEIALHSGEEPHAEEHLKGDFYESEHGINIDLNINNHLIAKEMEHNTVCAAGTSPVGEIGEEKILPTSETKQRTVLDTYPGVSEADAGETLSSTGP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SON
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 24872507)
• Control Peptide:

  SON Protein (NBP1-88706PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24872507).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SON Antibody

• BASS1C21orf50
• Bax antagonist selected in saccharomyces 1
• DBP5
• DBP-5
• FLJ33914
• KIAA1019SON3
• Negative regulatory element-binding protein
• NREBPNRE-binding protein
• Protein DBP-5
• protein SON
• SON DNA binding protein
• SON DNA-binding protein, KIAA101910FLJ21099

Background

SON - SON DNA binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SON Antibody (NBP1-88706)(4)

Publications using NBP1-88706

• Penev A, Bazley A, Shen M et al. Alternative splicing is a developmental switch for hTERT expression Mol Cell 2021-04-14 [PMID: 33852895]
• Ammon T, Mishra SK, Kowalska K et al. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol 2014-08-01 [PMID: 24872507] (WB, Human)
• Uhlen M, Oksvold P, Algenas C et al. Antibody-based Protein Profiling of the Human Chromosome 21. Mol Cell Proteomics 2012-03-01 [PMID: 22042635]
• Fuchs F, Pau G, Kranz D et al. Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Mol Syst Biol 2010-06-01 [PMID: 20531400]

Review for SON Antibody (NBP1-88706) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Agbolahan Sofela WB Human 04/11/2019

Summary

• Application: Western Blot
• Sample Tested: Tissue Lysates
• Species: Human
• Lot: B97068

Bioinformatics

• Gene Symbol: SON