![Western Blot: SON Antibody [NBP1-88706] - Analysis using Anti-SON antibody NBP1-88706 (A) shows similar pattern to independent antibody NBP2-55411 (B).](http://images.novusbio.com/fullsize2/SON-Antibody-Western-Blot-NBP1-88706-img0007.jpg "Western Blot: SON Antibody [NBP1-88706] - Analysis using Anti-SON antibody NBP1-88706 (A) shows similar pattern to independent antibody NBP2-55411 (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: TEVAIESTPMILESSIMSSHVMKGINLSSGDQNLAPEIGMQEIALHSGEEPHAEEHLKGDFYESEHGINIDLNINNHLIAKEMEHNTVCAAGTSPVGEIGEEKILPTSETKQRTVLDTYPGVSEADAGETLSSTGP |
| Specificity | Specificity of human SON antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SON |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 24872507) |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publications using NBP1-88706 | Applications | Species |
|---|---|---|
| Ammon T, Mishra SK, Kowalska K et al. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol 2014 Aug [PMID: 24872507] (WB, Human) | WB | Human |
| Uhlen M, Oksvold P, algenas C et al. Antibody-based protein profiling of the human chromosome 21. Mol Cell Proteomics 2012 Mar [PMID: 22042635] | ||
| Fuchs F, Pau G, Kranz D et al. Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Mol Syst Biol 2010 Jun [PMID: 20531400] | ||
| Penev A, Bazley A, Shen M et al. Alternative splicing is a developmental switch for hTERT expression bioRxiv Jan 1 2020 (WB) | WB |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Anonymous |
WB | Human | 04/11/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for SON Antibody (NBP1-88706)Discover more about diseases related to SON Antibody (NBP1-88706).
| Pathways for SON Antibody (NBP1-88706)View related products by pathway.
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PTMs for SON Antibody (NBP1-88706)Learn more about PTMs related to SON Antibody (NBP1-88706).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SON |
![Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/SON-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88706-img0006.jpg "Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize2/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0010.jpg "Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize2/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0008.jpg "Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize2/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0009.jpg "Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.](http://images.novusbio.com/fullsize2/SON Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0011.jpg "Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.")
![Western Blot: GLE1 Antibody [NBP1-76975] - Mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/images2/GLE1-Antibody-Western-Blot-NBP1-76975-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GLE1 Antibody [NBP1-76975] - Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 5 ug/mL.](http://images.novusbio.com/images2/GLE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76975-img0004.jpg)
![Immunohistochemistry-Paraffin: GLE1 Antibody [NBP1-76975] - Mouse brain tissue with Gle1 antibody at 2.5 ug/ml.](http://images.novusbio.com/images2/GLE1-Antibody-Immunohistochemistry-Paraffin-NBP1-76975-img0002.jpg)
![Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Niemann-Pick-C1-Antibody-Knockout-Validated-NB400-148-img0014.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/images2/Niemann-Pick-C1-Antibody-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: Niemann-Pick C1 Antibody [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Niemann-Pick-C1-Antibody-Western-Blot-NB400-148-img0015.jpg)
![Western Blot: RAE1 Antibody [NB100-1894] - Staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/RAE1-Antibody-Western-Blot-NB100-1894-img0003.jpg)
![Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/RAE1-Antibody-Immunohistochemistry-Paraffin-NB100-1894-img0002.jpg)
![Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/RAE1-Antibody-Immunohistochemistry-Paraffin-NB100-1894-img0001.jpg)
![Western Blot: NXF1 Antibody [NBP1-31649] - Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/NXF1 Antibody-Western Blot-NBP1-31649-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NXF1 Antibody [NBP1-31649] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/images2/NXF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31649-img0008.jpg)
![Immunohistochemistry-Paraffin: NXF1 Antibody [NBP1-31649] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/NXF1 Antibody-Immunohistochemistry-Paraffin-NBP1-31649-img0006.jpg)
![Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipobeta-depleted NIH 3T3 cytoplasmic lysate. Ponceau-stained full membrane (below). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.](http://images.novusbio.com/images2/DDX19B-Antibody-Western-Blot-NB100-760-img0010.jpg)
![Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/images2/DDX19B-Antibody-Immunohistochemistry-NB100-760-img0006.jpg)
![Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/images2/DDX19B-Antibody-Western-Blot-NB100-760-img0005.jpg)
![Western Blot: GLS2 Antibody [NBP1-76544] - Glutaminase protein expression in PC3 and PC3M cell lines before/after CB-839 treatment. PC3 & PC3M cells were treated (+) or not treated (-) with GLS1 inhibitor CB-839 (1 uM) and their expression of GLS1 & GLS2 proteins determined by Western blot. Antibodies specific for GAC & KCA were utilized on two replicate samples, while GLS2 expression was determined on three. PC3 cell lysates expressed GLS2 more than PC3M cell lysates regardless of CB-839 treatment. The difference in GLS2 expression was significant in the untreated cells (P?<?0.03) but because of high variability the difference was not significant in the drug-treated cells. Levels of GLS1 (GAC and KGA) were similar in all cell lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-16327-z), licensed under a CC-BY licence.](http://images.novusbio.com/images2/GLS2 Antibody-Western Blot-NBP1-76544-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.](http://images.novusbio.com/images2/GLS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76544-img0007.jpg)
![Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.](http://images.novusbio.com/images2/GLS2-Antibody-Immunohistochemistry-NBP1-76544-img0003.jpg)
![Western Blot: NUP214 Antibody [NB100-2244] - Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/images2/NUP214-Antibody-Western-Blot-NB100-2244-img0009.jpg)
![Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/NUP214-Antibody-Immunohistochemistry-Paraffin-NB100-2244-img0011.jpg)
![Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/NUP214-Antibody-Immunohistochemistry-Paraffin-NB100-2244-img0010.jpg)
![SDS-Page: Recombinant Human AGA Protein [NBP2-52631]](http://images.novusbio.com/images2/AGA-Recombinant-Protein-SDS-Page-NBP2-52631-img0001.jpg)
![Western Blot: Vitamin D BP Antibody [NBP1-88027] - Equine adult and embryonic liver cell lysates. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/Vitamin-D-BP-Antibody-Western-Blot-NBP1-88027-img0009.jpg)
![Immunohistochemistry-Paraffin: Vitamin D BP Antibody [NBP1-88027] - Staining of human liver shows cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/images2/Vitamin-D-BP-Antibody-Immunohistochemistry-Paraffin-NBP1-88027-img0006.jpg)
![Western Blot: Vitamin D BP Antibody [NBP1-88027] - Analysis using Anti-GC antibody NBP1-88027 (A) shows similar pattern to independent antibody NBP1-88028 (B).](http://images.novusbio.com/images2/Vitamin D BP Antibody-Western Blot-NBP1-88027-img0008.jpg)
![Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85296] - Staining of human liver, lung, lymph node and testis using Anti-HSD17B4 antibody NBP1-85296 (A) shows similar protein distribution across tissues to independent antibody NBP1-85297 (B).](http://images.novusbio.com/images2/HSD17B4-Antibody-Immunohistochemistry-Paraffin-NBP1-85296-img0009.jpg)
![Western Blot: HSD17B4 Antibody [NBP1-85296] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/HSD17B4-Antibody-Western-Blot-NBP1-85296-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody [NBP1-85296] - Staining of human cell line A-431 shows localization to peroxisomes. Antibody staining is shown in green.](http://images.novusbio.com/images2/HSD17B4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85296-img0007.jpg)
![Western Blot: D Box Binding Protein Antibody [NBP2-83996] - Host: Rabbit. Target Name: DBP. Sample Tissue: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/images2/D Box Binding Protein Antibody-Western Blot-NBP2-83996-img0003.jpg)
![Immunohistochemistry: D Box Binding Protein Antibody [NBP2-83996] - Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-DBP antibody](http://images.novusbio.com/images2/D-Box-Binding-Protein-Antibody-Immunohistochemistry-NBP2-83996-img0002.jpg)
![Immunohistochemistry: D Box Binding Protein Antibody [NBP2-83996] - Human Heart](http://images.novusbio.com/images2/D-Box-Binding-Protein-Antibody-Immunohistochemistry-NBP2-83996-img0001.jpg)
![Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.](http://images.novusbio.com/images2/ZBTB7A-Pokemon-Antibody-Western-Blot-NBP2-23673-img0005.jpg)
![Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of human skin using Pokemon antibody at 1:100.](http://images.novusbio.com/images2/ZBTB7A-Pokemon-Antibody-Immunohistochemistry-Paraffin-NBP2-23673-img0004.jpg)
![Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/gamma-Synuclein-Antibody-Western-Blot-NBP1-31209-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - Synuclein-gamma Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.](http://images.novusbio.com/images2/Synuclein-gamma-Antibody-Immunocytochemistry-NBP1-31209-img0006.jpg)
![Immunohistochemistry-Paraffin: Synuclein-gamma Antibody [NBP1-31209] - Paraffin-embedded mouse fore brain, gamma Synuclein antibody dilution: 1:500.](http://images.novusbio.com/images2/Synuclein-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-31209-img0007.jpg)
![Western Blot: Nab2 Antibody [NBP2-19462] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/images2/Nab2 Antibody-Western Blot-NBP2-19462-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nab2 Antibody [NBP2-19462] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Nab2 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19462-img0002.jpg)
![Immunohistochemistry-Paraffin: Nab2 Antibody [NBP2-19462] - Mouse fore brain. NAB2 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/images2/Nab2-Antibody-Immunohistochemistry-Paraffin-NBP2-19462-img0003.jpg)
![Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.](http://images.novusbio.com/images2/RUNX1T1-ETO-Antibody-Western-Blot-NBP2-55747-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/RUNX1T1-ETO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55747-img0001.jpg)
![Immunohistochemistry-Paraffin: Secretin R Antibody [NBP1-86125] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/images2/Secretin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-86125-img0005.jpg)
![Immunohistochemistry-Paraffin: Secretin R Antibody [NBP1-86125] - Staining of human pancreas shows strong membranous positivity in interlobular ducts.](http://images.novusbio.com/images2/Secretin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-86125-img0002.jpg)
![Immunohistochemistry-Paraffin: Secretin R Antibody [NBP1-86125] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/images2/Secretin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-86125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GCFC1 Antibody [NBP1-86818] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/images2/GCFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86818-img0005.jpg)
![Immunohistochemistry-Paraffin: GCFC1 Antibody [NBP1-86818] - Staining of human kidney shows strong cytoplasmic positivity in tubular cells.](http://images.novusbio.com/images2/GCFC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86818-img0006.jpg)
![Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/images2/DDX56-Antibody-(4C5)-Western-Blot-H00054606-M05-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/DDX56-Antibody-(4C5)-Immunocytochemistry-Immunofluorescence-H00054606-M05-img0007.jpg)
![Immunohistochemistry-Paraffin: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/images2/DDX56-Antibody-(4C5)-Immunohistochemistry-Paraffin-H00054606-M05-img0009.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")