Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate ...read more
Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB
Biological Strategies: Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing ...read more
Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. ...read more
The immunogen recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269).
Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%), Guinea Pig (100%), Orangutan (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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