DDX19B Antibody

Images

 
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate ...read more
Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB
Biological Strategies: Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; ...read more
Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing ...read more
Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Gp, Pm-Or, Xp, ZeSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

DDX19B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%), Guinea Pig (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX19B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2- 5 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-760 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX19B Antibody

  • ATP-dependent RNA helicase DDX19
  • DBP5ATP-dependent RNA helicase DDX19B
  • DDX19DEAD-box protein 5
  • DEAD (Asp-Glu-Ala-As) box polypeptide 19
  • DEAD (Asp-Glu-Ala-As) box polypeptide 19B
  • DEAD box protein 19B
  • DEAD box RNA helicase DEAD5
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)
  • DEAD-box RNA helicase DEAD5
  • EC 3.6.1
  • EC 3.6.4.13
  • RNAh
  • yeast Dbp5 homolog

Background

DDX19 is an ATP-dependent RNA helicase involved in mRNA export from the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-760
Species: Hu, Mu, Rt, Bv, Gp, Pm-Or, Xp, Ze
Applications: WB, IHC, IHC-P, IP

Publications for DDX19B Antibody (NB100-760)(4)

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Bioinformatics

Gene Symbol DDX19B
Entrez
Uniprot