DDX19B Antibody

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 binds directly to MKL1 RPEL domain. Recruitment of Ipobeta and Ddx19 from Ipobeta-depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (-), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipobeta-depleted NIH 3T3 cytoplasmic lysate. Ponceau-stained full membrane (below). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Immunohistochemistry: DDX19B Antibody [NB100-760] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Western Blot: DDX19B Antibody [NB100-760] - Detection of Human and Mouse. Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25-10 uM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms6978), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Bv, Gp, Pm-Or, Xp, Ze
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX19B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269).
• Predicted Species: Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%), Guinea Pig (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX19B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2- 5 ug/mg lysate
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX19B Antibody

• ATP-dependent RNA helicase DDX19
• DBP5ATP-dependent RNA helicase DDX19B
• DDX19DEAD-box protein 5
• DEAD (Asp-Glu-Ala-As) box polypeptide 19
• DEAD (Asp-Glu-Ala-As) box polypeptide 19B
• DEAD box protein 19B
• DEAD box RNA helicase DEAD5
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)
• DEAD-box RNA helicase DEAD5
• EC 3.6.1
• EC 3.6.4.13
• RNAh
• yeast Dbp5 homolog

Background

DDX19 is an ATP-dependent RNA helicase involved in mRNA export from the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX19B Antibody (NB100-760)(4)

Publications using NB100-760

• Zhang K, Zhang Y, Xue J et al. DDX19 Inhibits Type I Interferon Production by Disrupting TBK1-IKKe-IRF3 Interactions and Promoting TBK1 and IKKe Degradation Cell Rep Jan 29 2019 [PMID: 30699353] (WB, Human)
• Diot C, Fournier G, Dos Santos M et al. Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs Sci Rep Sep 22 2016 [PMID: 27653209] (WB, Human)
• Rajakyla EK, Viita T, Kyheroinen S et al. RNA export factor Ddx19 is required for nuclear import of the SRF coactivator MKL1 Nat Commun. 2015 Jan 14 [PMID: 25585691] (WB, Human, Mouse)
• Zolotukhin AS, Uranishi H, Lindtner S et al. Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Nucleic Acids Res 2009 Nov [PMID: 19786495]

Bioinformatics

• Gene Symbol: DDX19B
• Entrez:
  • 11269(Human)
  • 234733(Mouse)
• Uniprot:
  • NP_009173.1(Human)