GLE1 Antibody - BSA Free

Western Blot: GLE1 Antibody [NBP1-76975] - Mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: GLE1 Antibody - BSA Free [NBP1-76975] - Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 5 ug/mL.

Immunohistochemistry: GLE1 Antibody - BSA Free [NBP1-76975] - Immunohistochemistry of GLE1 in mouse brain tissue with GLE1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GLE1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Gle1. The immunogen is located within amino acids 540 - 590 of Gle1. Amino Acid Squence: VKDSKVEQQDNFLKR
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLE1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  GLE1 Peptide (NBP1-76975PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for GLE1 Antibody - BSA Free

• GLE1 (yeast homolog)-like, RNA export mediator
• GLE1 RNA export mediator homolog (yeast)
• GLE1LGLE1 RNA export mediator-like (yeast)
• GLE1-like protein
• GLE1-like, RNA export mediator
• hGLE1GLE1 RNA export mediator (yeast)
• LCCS
• LCCS1
• lethal congenital contracture syndrome 1
• nucleoporin GLE1

Background

The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLE1
• Uniprot:
  • NP_001003722(Human)