Somatostatin Antibody (906552) [Unconjugated]

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="Somatostatin was detected in immersion fixed human embryonic stem cells differentiated to pancreatic cells using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

Somatostatin was detected in immersion fixed human embryonic stem cells differentiated to pancreatic cells using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Somatostatin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Somatostatin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Somatostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Somatostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Somatostatin was detected in immersion fixed TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Somatostatin was detected in immersion fixed TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

MAB14171), and a Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358), followed by secondary antibody staining with the NorthernLights NL493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009; green) and NorthernLights NL557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013; red). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid." > MAB14171), and a Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358), followed by secondary antibody staining with the NorthernLights NL493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009; green) and NorthernLights NL557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013; red). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid." title="iPSC-derived beta islets were fixed, frozen, and sectioned. Sections were stained with a Mouse Anti-Human C-peptide Monoclonal Antibody (Catalog # MAB14171), and a Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358), followed by secondary antibody staining with the NorthernLights NL493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009; green) and NorthernLights NL557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013; red). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid." />

iPSC-derived beta islets were fixed, frozen, and sectioned. Sections were stained with a Mouse Anti-Human C-peptide Monoclonal Antibody (Catalog # <a class=

• Reactivity: Hu, Mu
• Applications: IHC, ICC/IF
• Clone: 906552
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Somatostatin Antibody (906552) [Unconjugated] Summary

• Immunogen: KLH-coupled human Somatostatin peptide
  Accession # P61278
• Specificity: Detects human Somatostatin in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: SST
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Somatostatin Antibody (906552) [Unconjugated]

• Growth hormone release-inhibiting factor
• SMST
• SOM
• Somatostatin
• somatostatin-14
• somatostatin-28
• SRIF
• SST

Background

Somatostatin (SST), also known as SMST, is a 116 amino acid (aa) peptide hormone protein and shares 96% aa identity with mouse and rat SST. It exists as a preproprotein that is cleaved into a proprotein form and then subsequently into 14 aa and 28 aa active peptide forms. SST is secreted by many tissues throughout the body and inhibits the secretion of many other hormones. Some of its major roles are inhibiting growth hormone secretion by the pituitary gland, insulin and glucagon secretion in pancreatic islets, and secretion of gastrointestinal tract hormones such as gastrin. Additionally, SST has been shown to have neuromodulatory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Somatostatin Antibody (MAB2358)(4)

Publications using MAB2358

• Ng, XW;DiGruccio, MR;Kong, C;Lee, J;Piston, DW; Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans bioRxiv : the preprint server for biology 2024-10-30 [PMID: 39554053] (Immunohistochemistry, Mouse)
• Marito Hayashi, Judith A Kaye, Ella R Douglas, Narendra R Joshi, Fiona M Gribble, Frank Reimann et al. Enteroendocrine cell lineages that differentially control feeding and gut motility eLife 2/22/2023 [PMID: 36810133]
• Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism 5/1/2021 [PMID: 34081913]
• HJ Rhee, AH Shaib, K Rehbach, C Lee, P Seif, C Thomas, E Gideons, A Guenther, T Krutenko, M Hebisch, M Peitz, N Brose, O Brüstle, JS Rhee An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons Cell Rep, 2019-05-14;27(7):2212-2228.e7. 2019-05-14 [PMID: 31091457] (ICC, Human)

Bioinformatics

• Gene Symbol: SST
• Uniprot:
  • P61278()