Soluble Liver/Pancreas Antigen Antibody - BSA Free

Western Blot: Soluble Liver/Pancreas Antigen Antibody [NBP2-94530] - Analysis of extracts of various cell lines, using Soluble Liver/Pancreas Antigen at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3s.

Immunocytochemistry/ Immunofluorescence: Soluble Liver/Pancreas Antigen Antibody - BSA Free [NBP2-94530] - Immunofluorescence analysis of U2OS cells using Soluble Liver/Pancreas Antigen antibody (A7103). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Soluble Liver/Pancreas Antigen Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human SEPSECS (NP_058651.3). STTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPSECS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Soluble Liver/Pancreas Antigen Antibody - BSA Free

• EC 2.9.1.2
• EC 2.9.1.n1
• Liver-pancreas antigen
• LPDKFZp434B1417
• MGC161491
• O-phosphoseryl-tRNA(Sec) selenium transferase
• Sec synthase
• Selenocysteine synthase
• Selenocysteinyl-tRNA(Sec) synthase
• Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
• SepSecS
• Sep-tRNA:Sec-tRNA synthase
• SLA/LP autoantigen
• SLA/LP
• SLA-p35
• SLAUGA suppressor tRNA-associated protein
• Soluble liver antigen
• soluble liver antigen/liver pancreas antigen
• tRNA(Ser/Sec)-associated antigenic protein
• TRNP48

Background

The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor (Palioura et al., 2009 (PubMed 19608919)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPSECS