Rat Soluble CD25/IL-2R alpha - soluble ELISA Kit (Colorimetric)

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ELISA: Rat Soluble CD25/IL-2R alpha  - soluble ELISA Kit (Colorimetric) [NBP2-79697] - Samples were spiked with high concentrations of Rat Soluble CD25/IL-2R alpha and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Rat Soluble CD25/IL-2R alpha  - soluble ELISA Kit (Colorimetric) [NBP2-79697] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, and other biological fluids
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

Rat Soluble CD25/IL-2R alpha - soluble ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat IL-2sR alpha/CD25 in samples. No Significant cross-reactivity or interference between Rat IL-2sR alpha/CD25 and analogues was observed.
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.57%
Intra-Assay
CV% < 5.07%
Spike Recovery
88-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Soluble CD25/IL-2R alpha - soluble ELISA Kit (Colorimetric)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 receptor subunit alpha
  • IL-2R subunit alpha
  • IL2R
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • Soluble CD25/IL-2R alpha
  • TAC antigen
  • TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Soluble CD25/IL-2R alpha ELISA Kit (NBP2-79697). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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