SOHLH2 Antibody - BSA Free

Western Blot: SOHLH2 Antibody [NBP2-20453] - Sample (30 ug of whole cell lysate) A: NT2D1 B: MCF-7 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SOHLH2 Antibody [NBP2-20453] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: SOHLH2 Antibody [NBP2-20453] - Ca922 xenograft, using SOHLH2 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SOHLH2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SOHLH2. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOHLH2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SOHLH2 Antibody - BSA Free

• bHLHe81
• FLJ57222
• spermatogenesis and oogenesis specific basic helix-loop-helix 2
• spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
• TEB1

Background

Probable transcription factor, which may be involved in spermatogenesis and oogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SOHLH2 Antibody (NBP2-20453)(1)

Publication using NBP2-20453

• Zhang R, Zhang X, Zhang W et al. Sohlh2 regulates the stemness and differentiation of colon cancer stem cells by downregulating LncRNA-H19 transcription Molecular cancer research : MCR 2022-10-26 [PMID: 36287177] (WB, Human)

Bioinformatics

• Gene Symbol: SOHLH2
• Uniprot:
  • Q9NX45(Human)