Lhx8 Antibody - BSA Free

Western Blot: Lhx8 Antibody [NBP1-32840] - (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:5000

Immunoprecipitation: Lhx8 Antibody [NBP1-32840] - 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of LIM homeobox protein 8 protein by 2 ug of LIM homeobox protein 8 antibody 10% SDS-PAGE The immunoprecipitated LIM homeobox protein 8 protein was detected by LIM homeobox protein 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lhx8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lhx8 Antibody - BSA Free (NBP1-32840) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Lhx8 Recombinant protein.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LHX8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%), Rat (88%), Pig (82%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Lhx8 Antibody - BSA Free

• LIM homeobox 8
• LIM homeobox protein 8

Background

Members of the LIM homeobox gene family, such as LHX8, encode transcription regulators that share common structuralfeatures. They all contain 2 tandemly repeated cysteine-rich double-zinc finger motifs, called LIM domains, inaddition to a homeodomain. The homeodomain is a DNA-binding domain, and the LIM domains are essential for regulatingthe activity of these molecules by interacting with other proteins. Members of the LIM homeobox gene family arerequired for the patterning or the specification and differentiation of different cell types during embryonicdevelopment (Zhao et al., 1999 (PubMed 10611327)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: LHX8