SODD/BAG4 Antibody - BSA Free

Western Blot: SODD/BAG4 Antibody [NBP1-76897] - Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: SODD/BAG4 Antibody - BSA Free [NBP1-76897] - Immunofluorescence of SODD/BAG4 in Hela cells with SODD/BAG4 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: SODD/BAG4 Antibody - BSA Free [NBP1-76897] - Immunocytochemistry of SODD/BAG4 in HeLa cells with SODD/BAG4 antibody at 5 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SODD/BAG4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SODD/BAG4 Antibody - BSA Free (NBP1-76897) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-SODD/BAG4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to amino acids near the amino terminus of human SODD. The immunogen is located within the first 50 amino acids of SODD. Amino Acid Squence: SALRRSGYGPSDGPS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG4
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: An approximately 60 kDa band can be detected in Western Blot.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SODD/BAG4 Peptide (NBP1-76897PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for SODD/BAG4 Antibody - BSA Free

• BAG4
• BAG-4
• BAG-family molecular chaperone regulator-4
• Bcl-2-associated athanogene 4
• BCL2-associated athanogene 4
• Silencer of death domains
• SODD
• SODDBAG family molecular chaperone regulator 4

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SODD/BAG4 Antibody (NBP1-76897)(1)

Publication using NBP1-76897

• Gupta MK, Tahrir FG, Knezevic T et al. GRP78 Interacting Partner Bag5 Responds to ER Stress and Protects Cardiomyocytes from ER Stress-Induced Apoptosis. J. Cell. Biochem. 2016-01-05 [PMID: 26729625] (WB, Rat)

Bioinformatics

• Gene Symbol: BAG4
• Uniprot:
  • AF111116(Human)