Human SOD3/EC-SOD ELISA Kit (Colorimetric)

Product Discontinued

Human SOD3/EC-SOD ELISA Kit (Colorimetric) Summary

• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Cu/ZnSOD positive serum. There was no crossreactivity detected.
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.04 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.8%
• Intra-Assay: CV% < 5.1%
• Spike Recovery: The recovery ranged from 89% to 108% with an overall mean recovery of 98%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SOD3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human Cu/ZnSOD ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cu/ZnSOD

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human SOD3/EC-SOD ELISA Kit (Colorimetric)

• ECSOD
• EC-SOD
• EC-SODEC 1.15.1.1
• extracellular superoxide dismutase [Cu-Zn]
• MGC20077
• SOD3
• superoxide dismutase 3, extracellular

Background

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Secreted protein; extracellular space. Note=99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in quilibrium between the plasma and the endothelium. TISSUE SPECIFICITY: Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. POLYMORPHISM: The variant Gly-231 which is found in about 2.2% of individual displays a 10-fold increased plasma EC-SOD content due to reduced heparin-binding affinity and thus the impairment of its binding ability to endothelial cell surface. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SOD3