SOD2/Mn-SOD Antibody (JJ089-02)

Western Blot: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of SOD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse brain tissue lysateLane 2: SH-SY5Y cell lysate Lane 3: mouse heart tissue lysate

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of paraffin-embedded mouse liver tissue using anti-SOD2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of paraffin-embedded human kidney tissue using anti-SOD2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of paraffin-embedded human liver tissue using anti-SOD2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of paraffin-embedded mouse colon tissue using anti-SOD2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (JJ089-02) [NBP2-67879] - Analysis of paraffin-embedded mouse heart tissue using anti-SOD2 antibody. Counter stained with hematoxylin.

Product Discontinued

SOD2/Mn-SOD Antibody (JJ089-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SOD2/Mn-SOD aa 1-50 / 222. (SwissProt: P04179 Human; SwissProt: P09671 Mouse; SwissProt: P07895 Rat)
• Localization: Mitochondrion matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SOD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SOD2/Mn-SOD Antibody (JJ089-02)

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOD2