SOCS-7/Nck/NAP4 Antibody (519106) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 519106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

SOCS-7/Nck/NAP4 Antibody (519106) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human SOCS-7
  His351-Thr581
  Accession # O14512
• Specificity: Detects endogenous human SOCS-7 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOCS7
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for SOCS-7/Nck/NAP4 Antibody (519106) [CoraFluor™ 1]

• NAP4
• NAP-4
• NAP4SOCS-7
• Nck
• Nck, Ash and phospholipase C binding protein
• Nck, Ash and phospholipase C gamma-binding protein
• NCKAP4
• Nck-associated protein 4
• SOCS6
• SOCS7
• SOCS-7
• suppressor of cytokine signaling 7

Background

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS7