Snx6 Overexpression Lysate (Native)

Images

 
Western Blot: Snx6 Overexpression Lysate (Adult Normal) [NBL1-16320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Snx6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Snx6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200981 and based on accession number NM_021249. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sorting nexin 6 (SNX6), transcript variant 1, mRNA.
Gene
SNX6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Snx6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Snx6 Overexpression Lysate (Native)

  • MGC3157
  • MSTP010
  • sorting nexin 6
  • sorting nexin-6
  • TFAF2
  • TRAF4-associated factor 2
  • tumor necrosis factor receptor-associated factor 4(TRAF4)-associated factor 2

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript, and differs in the 5' UTR. The resulting isoform (a) has a shorter N-terminus compared to isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SNX6