Snx6 Overexpression Lysate

Western Blot: Snx6 Overexpression Lysate (Adult Normal) [NBL1-16320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Snx6.

• Reactivity: Hu
• Applications: WB

Snx6 Overexpression Lysate Summary

• Description: Transient overexpression lysate of sorting nexin 6 (SNX6), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200981 and based on accession number NM_021249. The protein contains a C-MYC/DDK Tag.
• Gene: SNX6

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 33.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Snx6 Overexpression Lysate

• MGC3157
• MSTP010
• sorting nexin 6
• sorting nexin-6
• TFAF2
• TRAF4-associated factor 2
• tumor necrosis factor receptor-associated factor 4(TRAF4)-associated factor 2

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript, and differs in the 5' UTR. The resulting isoform (a) has a shorter N-terminus compared to isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SNX6