BRMS1 Antibody (2D4-2G11) - Azide and BSA Free

Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - BRMS1 monoclonal antibody (M01), clone 2D4-2G11. Analysis of BRMS1 expression in human liver.

Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 expression in transfected 293T cell line by BRMS1 monoclonal antibody (M01), clone 2D4-2G11.Lane 1: BRMS1 transfected lysate (Predicted MW: 28.5 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 transfected lysate using anti-BRMS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BRMS1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Detection limit for recombinant GST tagged BRMS1 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 2D4-2G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BRMS1 Antibody (2D4-2G11) - Azide and BSA Free Summary

• Immunogen: BRMS1 (AAH09834, 1 a.a. ~ 246 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
• Specificity: BRMS1 - breast cancer metastasis suppressor 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRMS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against tissue lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BRMS1 Antibody (2D4-2G11) - Azide and BSA Free

• breast cancer metastasis suppressor 1
• breast cancer metastasis-suppressor 1
• DKFZP564A063

Background

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRMS1 Antibody (H00025855-M01)(10)

Publications using H00025855-M01

• Ventura BV, Quezada C, Maloney SC et al. Expression of the metastasis suppressor BRMS1 in uveal melanoma. Ecancermedicalscience. 2014-03-11 [PMID: 24688598]
• Liu Y, Mayo MW, Nagji AS et al. BRMS1 Suppresses Lung Cancer Metastases through an E3 Ligase Function on Histone Acetyltransferase p300. Cancer Res. 2013-02-07 [PMID: 23269275]
• Cui RX, Liu N, He QM et al. Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival. BMC Cancer. 2012-08-29 [PMID: 22931099]
• Kim B, Nam HJ, Pyo KE et al. Breast Cancer Metastasis Suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex. Biochem Biophys Res Commun. 2011-11-09 [PMID: 22085717]
• Nagji AS, Liu Y, Stelow EB et al. BRMS1 transcriptional repression correlates with CpG island methylation and advanced pathologic stage in non-small cell lung cancer. J Pathol 2010-05-11 [PMID: 20455258]
• Smith PW, Liu Y, Siefert SA et al. Breast cancer metastasis suppressor 1 (BRMS1) suppresses metastasis correlates with improved patient survival in non-small cell lung cancer. Cancer Lett;276(2):196-203. 2009-04-18 [PMID: 19111386]
• Rivera J, Megias D, Bravo J. Proteomics-Based Strategy To Delineate the Molecular Mechanisms of the Metastasis Suppressor Gene BRMS1. J Proteome Res;6(10):4006-18. 2007-10-01 [PMID: 17854218]
• Arai T, Oda T et al. TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration amyotrophic lateral sclerosis. Biochem Biophys Res Commun;351(3):602-11. 2006-12-22 [PMID: 17084815]
• Liu Y, Smith PW, Jones DR. Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing HDAC1-mediated deacetylation of RelA/p65 promoting apoptosis. Mol Cell Biol;26(23):8683-96. 2006-12-01 [PMID: 17000776]
• Hudelist G, Singer CF, Pischinger KI et al. Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium. Proteomics 2006-03-01 [PMID: 16470630] (WB, Human)

Bioinformatics

• Gene Symbol: BRMS1
• Entrez:
  • 25855(Human)
• OMIM:
  • 606259(Human)
• Uniprot:
  • AAH09834(Human)