VPS26A Antibody (CL2287) - BSA Free

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.

Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-VPS26A monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % inten

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U251 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in HeLa cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human cell line A431 showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in MCF7 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U2OS cell line with Anti-VSP26A monoclonal antibody, showing distinct staining of endosomes in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL2287
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

VPS26A Antibody (CL2287) - BSA Free Summary

• Description: Novus Biologicals Mouse VPS26A Antibody (CL2287) - BSA Free (NBP2-36754) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-VPS26A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O75436, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (96%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VPS26A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  VPS26A Recombinant Protein Antigen (NBP2-36754PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for VPS26A Antibody (CL2287) - BSA Free

• FLJ12930
• HB58
• Hbeta58
• hVPS26
• PEP8A
• vacuolar protein sorting 26 (yeast homolog)
• vacuolar protein sorting 26 homolog A (S. pombe)
• vacuolar protein sorting 26 homolog A (yeast)
• vacuolar protein sorting-associated protein 26A
• Vesicle protein sorting 26A
• VPS26vacuolar protein sorting 26 (yeast)

Background

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS26A Antibody (NBP2-36754)(2)

Publications using NBP2-36754

• Simoes S, Guo J, Buitrago L et al. Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling Cell reports 2021-12-28 [PMID: 34965419]
• Aranda JF, Rathjen S, Johannes L, Fernandez-Hernando C. MiR-199a-5p attenuates retrograde transport and protects against toxin-induced inhibition of protein biosynthesis Mol. Cell. Biol. 2018-03-19 [PMID: 29555727] (WB, Human)

Bioinformatics

• Gene Symbol: VPS26A
• Uniprot:
  • O75436(Human)