SNX5 Antibody

Images

 
Western Blot: SNX5 Antibody [NB100-1362] - Analysis of SNX5 in Jurkat lysate (RIPA buffer, 35ug total protein per lane) using this antibody at 1.5 ug/ ml. Primary incubated for 1 hour. Detected using chemiluminescence.
Immunohistochemistry-Paraffin: SNX5 Antibody [NB100-1362] - (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SNX5 Antibody Summary

Immunogen
Peptide with sequence SLLQSCIDLFKNN corresponding to C-Terminus according to NP_055241.1, NP_689413.1.
Specificity
Both variants represent identical protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SNX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 48 kDa band observed in human kidney, Jurkat, and A549 lysates (calculated MW of 46.8 kDa band according to NP_055241.1). IHC-P: Human breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of human kidney and in trophoblasts of human placenta.
Positive Control
Jurkat Lysate (NB800-PC2)
SNX5 Lysate (NBL1-16318)
Publications
Read Publication using NB100-1362.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat. Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNX5 Antibody

  • FLJ10931
  • sorting nexin 5
  • sorting nexin-5

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein binds to fanconi anemia complementation group A protein, but its function is unknown. This gene results in two transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SNX5 Antibody (NB100-1362)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNX5
Entrez
Uniprot