SNX5 Antibody

Western Blot: SNX5 Antibody [NB100-1362] - Staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SNX5 Antibody [NB100-1362] - Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SNX5 Antibody Summary

• Immunogen: Peptide with sequence SLLQSCIDLFKNN corresponding to C-Terminus according to NP_055241.1, NP_689413.1.
• Specificity: Both variants represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human kidney, Jurkat, and A549 lysates (calculated MW of 46.8 kDa band according to NP_055241.1). IHC-P: Human breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of human kidney and in trophoblasts of human placenta.
• Control:

  Jurkat Whole Cell Lysate
  SNX5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX5 Antibody

• FLJ10931
• sorting nexin 5
• sorting nexin-5

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein binds to fanconi anemia complementation group A protein, but its function is unknown. This gene results in two transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX5 Antibody (NB100-1362)(1)

Publication using NB100-1362

• Otsuki T, Kajigaya S, Ozawa K, Liu JM. SNX5, a new member of the sorting nexin family, binds to the Fanconi anemia complementation group A protein. Biochem Biophys Res Commun 1999-11-30 [PMID: 10600472]

Bioinformatics

• Gene Symbol: SNX5
• Entrez:
  • 27131(Human)
  • 69178(Mouse)
• Uniprot:
  • NP_055241(Human)