SMYD3 Antibody (JM73-63)

Western Blot: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution.Positive control:Lane1: Rat kidney Lane2: PC-12Lane3: Hela Lane4: Mouse spleenLane5: NIH-3T3 Lane6: Rat spleen

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (JM73-63) [NBP2-75668] - Staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.

Flow Cytometry: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (JM73-63) [NBP2-75668] - Staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (JM73-63) [NBP2-75668] - Staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.

Product Discontinued

SMYD3 Antibody (JM73-63) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SMYD3 aa 251-300 / 428. (SwissProt: Q9H7B4 Human; SwissProt: Q9CWR2 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMYD3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMYD3 Antibody (JM73-63)

• bA74P14.1 (novel protein)
• bA74P14.1
• EC 2.1.1
• EC 2.1.1.43
• FLJ21080
• KMT3E
• MGC104324
• MYND domain containing 1
• SET and MYND domain containing 3
• SET and MYND domain-containing protein 3
• SMYD3
• Zinc finger MYND domain-containing protein 1
• zinc finger protein, subfamily 3A (MYND domain containing), 1
• ZMYND1
• ZNFN3A1

Background

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMYD3