Smoothened Antibody

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Western Blot: Smoothened Antibody [NB100-55392] - Western Blot: Smoothened Antibody [NB100-55392] - A) NB100-55392 and B) NB100-55397(0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Smoothened Antibody Summary

Immunogen
Peptide with sequence C-NLMDTELMDADSD corresponding to C-Terminus according to NP_005622.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMO
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 60 kDa band observed in human bone marrow lysates (calculated MW of 86.4 kDa band according to NP_005622.1). The observed molecular weight corresponds to earlier findings with NB100-55397, a product of different design reacting to a different part of the same protein.
Publications
Read Publication using NB100-55392.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smoothened Antibody

  • Gx
  • Protein Gx
  • SMOHseven transmembrane helix receptor
  • smoothened (Drosophila) homolog
  • smoothened homolog (Drosophila)
  • smoothened homolog

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smoothened Antibody (NB100-55392)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Smoothened Products

Bioinformatics Tool for Smoothened Antibody (NB100-55392)

Discover related pathways, diseases and genes to Smoothened Antibody (NB100-55392). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Smoothened Antibody (NB100-55392)

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Blogs on Smoothened.

The role of Smoothened in pulmonary pathologies
The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche...  Read full blog post.

Pancreatic Cancer Research Targets Hedgehog Signaling Pathway
The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact...  Read full blog post.

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Bioinformatics

Gene Symbol SMO
Entrez
Uniprot