Smoothelin Antibody (885424) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: Flow
• Clone: 885424
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Smoothelin Antibody (885424) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Smoothelin B
  Met1-Leu132
  Accession # P53814
• Specificity: Detects human Smoothelin B in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smoothelin Antibody (885424) [Alexa Fluor® 647]

• FLJ35365
• FLJ38597
• Smoothelin
• SMSMO
• SMTN

Background

Smoothelin (SMTN) is a cytoskeletal protein associated with stress fibers.  It is 917 amino acids (aa) in length and shares 77% and 82% aa identity with mouse and rat SMTN.  SMTN has two major isoforms, A and B with reported molecular weights of approximately 59 and 110 kDa, respectively.  Isoform A is associated with visceral smooth muscle cells while isoform B is specific to vascular smooth muscle.  Loss of SMTN has been associated with defects in intestinal smooth muscle cell contraction as well as increased arterial pressure and cardiac hypertrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smoothelin Antibody (IC8278R)(1)

Publication using IC8278R

• Y Qi, X Liang, H Guan, J Sun, W Yao RhoGDI1-Cdc42 Signaling Is Required for PDGF-BB-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells and Neointima Formation Biomedicines, 2021-09-06;9(9):. 2021-09-06 [PMID: 34572355] (IHC, Rat)

Bioinformatics

• Uniprot:
  • P53814()