SMCX Knockout 293T Cell Lysate

Images

 
There are currently no images for SMCX Lysate (NBP2-65611).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

SMCX Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2
Gene
KDM5C

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SMCX Knockout 293T Cell Lysate

  • DXS1272EMRXJ
  • EC 1.14.11
  • EC 1.14.11.-
  • Histone demethylase JARID1C
  • JARID1Clysine-specific demethylase 5C
  • Jumonji, AT rich interactive domain 1C (RBP2-like)
  • jumonji, AT rich interactive domain 1C
  • Jumonji/ARID domain-containing protein 1C
  • lysine (K)-specific demethylase 5C
  • MRXSJ
  • Protein SmcX
  • Protein Xe169
  • Smcx homolog, X chromosome
  • SMCXselected cDNA on X
  • Smcy homolog, X-linked (mouse)
  • Smcy homolog, X-linked
  • XE169JmjC domain-containing protein SMCX

Background

This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

22240002
Species: Hu
Applications: WB, ELISA
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
NB110-40499
Species: Hu, Mu
Applications: WB, IP
NB100-97821
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, IF
NBP1-80628
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-65171
Species: Hu
NBP1-87691
Species: Hu
Applications: WB, IHC, IHC-P
NB100-81657
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB100-485
Species: Hu
Applications: WB, IP
NB100-56326
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1762
Species: Hu, Mu, Pm
Applications: WB, Simple Western, ChIP, ELISA, IHC, IHC-P
NBP2-67451
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-82761
Species: Hu
Applications: IHC, IHC-P
NB100-204
Species: Hu, Mu, Xp
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
NB300-104
Species: Hu, Mu, Rt, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-85464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80582
Species: Hu
Applications: IHC, IHC-P

Publications for SMCX Lysate (NBP2-65611) (0)

There are no publications for SMCX Lysate (NBP2-65611).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SMCX Lysate (NBP2-65611) (0)

There are no reviews for SMCX Lysate (NBP2-65611). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SMCX Lysate (NBP2-65611) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for SMCX Lysate (NBP2-65611)

Discover related pathways, diseases and genes to SMCX Lysate (NBP2-65611). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SMCX Lysate (NBP2-65611)

Discover more about diseases related to SMCX Lysate (NBP2-65611).
 

Pathways for SMCX Lysate (NBP2-65611)

View related products by pathway.

PTMs for SMCX Lysate (NBP2-65611)

Learn more about PTMs related to SMCX Lysate (NBP2-65611).

Blogs on SMCX

There are no specific blogs for SMCX, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SMCX Antibody
NB100-55328
Synapsin I Antibody
NB300-104-100ul

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SMCX Knockout 293T Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol KDM5C