SMCX Knockout 293T Cell Lysate


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Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

SMCX Knockout 293T Cell Lysate Summary

Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2


Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Knockout 293T Cell
Life Stage


Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SMCX Knockout 293T Cell Lysate

  • DXS1272EMRXJ
  • EC 1.14.11
  • EC 1.14.11.-
  • Histone demethylase JARID1C
  • JARID1Clysine-specific demethylase 5C
  • Jumonji, AT rich interactive domain 1C (RBP2-like)
  • jumonji, AT rich interactive domain 1C
  • Jumonji/ARID domain-containing protein 1C
  • lysine (K)-specific demethylase 5C
  • Protein SmcX
  • Protein Xe169
  • Smcx homolog, X chromosome
  • SMCXselected cDNA on X
  • Smcy homolog, X-linked (mouse)
  • Smcy homolog, X-linked
  • XE169JmjC domain-containing protein SMCX


This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Gene Symbol KDM5C