ZFX Antibody - BSA Free

Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human kidney shows weak to moderate nuclear positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human lymph node shows moderate nuclear positivity in non-germinal center cells.

ChIP-Exo-Seq composite graph for Anti-ZFX (NBP1-80582) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu
• Applications: IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZFX Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZFX Antibody - BSA Free (NBP1-80582) is a polyclonal antibody validated for use in IHC. Anti-ZFX Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VSLAHCTVPDDVLASDITSASMSMPEHVLTGDSIHVSDVGHVGHVGHVEHVVHDSVVEAEIVTDPLTTDVVSEEVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZFX
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: IHC-P reported in scientific literature (PMID: 25355536). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ZFX Protein (NBP1-80582PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ZFX Antibody - BSA Free

• zinc finger protein, X-linked

Background

ZFX, also known as Zinc finger X-chromosomal protein, is a 91kDa 805 amino acid protein with a shorter 66kDa 576 isoform. The gene can be found in the nucleus where it encodes a member of the krueppel C2H2-type zinc-finger protein family. Current research is being conducted on ZFX in relation to the diseases azoospermia, prostate adenocarcinoma, premature ovarian failure and adenocarcinoma. ZFX is linked to the dosage compensation, sex determination, spermatogenesis, meiosis, cell cycle and fertilization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZFX Antibody (NBP1-80582)(4)

Publications using NBP1-80582

• Latif A, Fisher LE, Dundas AA et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing Advanced materials (Deerfield Beach, Fla.) 2022-11-28 [PMID: 36440539] (IHC-P, Human)
• Ma H, Yang F, Lian M et al. Dysregulation of zinc finger protein, X-linked (ZFX) impairs cell proliferation and induces apoptosis in human oral squamous cell carcinorma Tumour Biol. 2015-04-28 [PMID: 25916205] (IHC-P, Human)
• Yin J, Jiang Y, Wu H et al. Overexpression of ZFX and its involvement in squamous cell carcinoma of the tongue Oncol. Rep. 2015-01-01 [PMID: 25355536] (IHC-P, Human)
  
  Details:
  ZFX antibody used for IHC-P on tissues sections from human patients with squamous cell carcinoma of the tongue.
• Jiang H, Zhang L, Liu J et al. Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9. Cancer Gene Ther 2012-10-01 [PMID: 22898899]

Bioinformatics

• Gene Symbol: ZFX
• Entrez:
  • 7543(Human)
• Uniprot:
  • P17010(Human)