SMC6L1 Antibody (2E6) - Azide and BSA Free

Western Blot: SMC6L1 Antibody (2E6) [H00079677-M01] - SMC6L1 monoclonal antibody (M01), clone 2E6 Analysis of SMC6L1 expression in HeLa.

Immunohistochemistry-Paraffin: SMC6L1 Antibody (2E6) [H00079677-M01] - Analysis of monoclonal antibody to SMC6L1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

ELISA: SMC6L1 Antibody (2E6) [H00079677-M01] - Detection limit for recombinant GST tagged SMC6L1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, ChIP
• Clone: 2E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SMC6L1 Antibody (2E6) - Azide and BSA Free Summary

• Immunogen: SMC6L1 (AAH39828, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKRKEENFSSPKNAKRPRQEELEDFDKDGDEDECKGTTLTAAEVGIIESIHLKNFMCHSMLGPFKFGSNVNFVVGNNGSGKSAVLTALIVGLGGRAVATNRGSSLKGFV
• Specificity: SMC6L1 - SMC6 structural maintenance of chromosomes 6-like 1 (yeast)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMC6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SMC6L1 Antibody (2E6) - Azide and BSA Free

• FLJ22116
• hSMC6
• SMC protein 6
• SMC6 structural maintenance of chromosomes 6-like 1 (yeast)
• SMC6 structural maintenance of chromosomes 6-like 1
• SMC-6
• SMC6L1FLJ35534
• structural maintenance of chromosomes 6
• structural maintenance of chromosomes protein 6

Background

SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMC6L1 Antibody (H00079677-M01)(2)

Publications using H00079677-M01

• Wenjiao Z, Yuanping S, Changrun Z et al. In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance. Clin Transl Med. 2023-01-15 [PMID: 36627765]
• Gallego-Paez LM, Tanaka H, Bando M et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell. 2013-11-20 [PMID: 24258023]

Bioinformatics

• Gene Symbol: SMC6
• Entrez:
  • 79677(Human)
• OMIM:
  • 609387(Human)
• Uniprot:
  • AAH39828(Human)