SMARCAD1 Antibody

Images

 
Genetic Strategies: Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMARCAD1 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: SMARCAD1 Antibody [NBP1-85023] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in human cell line MOLT-4.
Western Blot: SMARCAD1 Antibody [NBP1-85023] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: SMARCAD1 Antibody [NBP1-85023] - Staining of human duodenum shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

SMARCAD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKK
Specificity
Specificity of human, mouse, rat SMARCAD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SMARCAD1 Protein (NBP1-85023PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMARCAD1 Antibody

  • ATP-dependent helicase 1
  • DKFZP762K2015
  • EC 3.6.1
  • EC 3.6.4.12
  • HEL1
  • hHEL1
  • KIAA1122ETL1DKFZp762K2015
  • subfamily a, containing DEAD/H box 1
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1
  • SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics Tool for SMARCAD1 Antibody (NBP1-85023)

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Bioinformatics

Gene Symbol SMARCAD1