Smad3 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

Smad3 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Smad3
  Ser2-Ala230
  Accession # P84022
• Specificity: Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad3 Antibody [Alexa Fluor® 700]

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Smad3 Antibody (AF3797N). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.