Smad2 [p Thr220] Antibody

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Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) ...read more
Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Smad2 [p Thr220] Antibody Summary

Immunogen
Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2 (NP_001003652.1).
Modification
p Thr220
Specificity
Smad2 (Phospho-Thr220) detects endogenous levels of Smad2 only when phosphorylated at threonine 220.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IHC-P reactivity reported in scientific literature (PMID: 23370707).
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-04982 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+) [pH 7.4] with 150mM NaCl and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smad2 [p Thr220] Antibody

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2
  • MADH2MGC34440
  • MADR2
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • pSMAD
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smox

Background

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad2 Antibody (NBP1-04982)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
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(1)
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Bioinformatics

Gene Symbol SMAD2
Entrez
Uniprot