Smad2 [p Thr220] Antibody

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Smad2 [p Thr220] Antibody Summary

• Immunogen: Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2 (NP_001003652.1).
• Modification: p Thr220
• Specificity: Detects endogenous levels of Smad2 only when phosphorylated at threonine 220.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: IHC-P reactivity reported in scientific literature (PMID: 23370707).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smad2 [p Thr220] Antibody

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• pSMAD
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2 Antibody (NBP1-04982)(1)

Publication using NBP1-04982

• Lee JM, Lee DJ, Bae SC, Jung HS. Abnormal liver differentiation and excessive angiogenesis in mice lacking Runx3. Histochem Cell Biol 2013-01-31 [PMID: 23370707] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SMAD2
• Entrez:
  • 4087(Human)
  • 17126(Mouse)
  • 29357(Rat)
• Uniprot:
  • Q15796(Human)